Bioinformatics Pilot Program Abstract: Stuart G. Gordon

Insights into Chronic anemia through Comparative Functional Analysis of Fish Gut
Dr. Stuart G. Gordon (PI), Padmanabhan Mahadevan, Naya Martin, Department of Biology, Presbyterian College

Members of the Channichthyidae family (the icefishes) are the only vertebrate group lacking the iron-containing oxygen transport metalloprotein hemoglobin. This mutant condition likely reducestheir need to extract iron from their diet. We hypothesize that the gut microbiome of icefishes will possess different functional capacity compared to their closely-related red-blooded counterparts and other teleosts, and that these microbial functions are likely to help balance iron acquisition in icefish hosts. We propose the sequencing and functional analysis of fish gut microbiomes (bacterial community). We currently have performed taxonomic analysis of the microbial species harbored in the guts of four different fish species, two each from temperate US coastal waters and Antarctic waters, by sequence analysis of 16s rRNA gene amplicons. We propose to sequence the total metagenomic DNA from the same fish samples to begin to characterize the functional (ie, enzymatic capabilities) of their respective gut microbiomes. This will involve one faculty member at PC, Stuart Gordon, one PC student, Naya Martin and collaborators. Sequencing of samples will be performed at MUSC's Proteogenomics facility. Collaborators at the University of Tampa will assist with data analysis.The proposed work will provide further preliminary data for a research proposal we are preparing and, in combination with the 16s data should provide results to report in a manuscript. Ms. Martin will report the results of her work at two PC researchsymposia, in July, 2016 and April, 2017.

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SC INBRE is funded by grant P20GM103499 from the National Institute of General Medical Sciences, National Institutes of Health

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