Bioinformatics Pilot Program Abstract: Wayne Carver

Effects of ethanol on myocyte-derived exosomal mRNA and miRNA content
Dr. Wayne E. Carver, Department of Cell Biology and Anatomy, University of South Carolina School of Medicine-Columbia

Chronic alcohol abuse has deleterious effects on most organs and organ systems. In the heart, long-term consumption of high levels of alcohol results in myocardial hypertrophy and fibrosis and eventually heart failure. Several studies have shown that these alterationsinvolveinteractions between the multiple cell types of the heart. Our preliminary data suggests that exosomes provide a mechanism of communication between myocytes and fibroblasts and that components of the myocyte-derived exosomes modify the response of fibroblasts to alcohol. Interestingly, exosomes fromcontrol myocytes are able to attenuate activation of fibroblasts by alcohol while exosomes from myocytes which are themselves treated with alcohol exacerbate alcohol-induced fibroblast activation. Based on this, we hypothesize that exosomal cargo of myocytes is altered in response to ethanol treatment and that this underlies the differential modulation of fibroblast activation by these exosomes. To test this, we propose to use mRNA and miRNA arrays to identify components that are differentially contained in exosomes from control and an alcohol-treated cardiac myocyte cell line. This will provide candidate mRNA and/or miRNAs that will be functionally evaluated in future proposals.Ultimately these candidate molecules may be targets for therapeutic intervention of damage caused by alcohol abuse.